Clinical Chemistry Diagnostic Exams

I.                  IDENTIFICATION: 

1. The major bile pigment.
2. The enzyme responsible for the conversion of B1 to B2.
3.  In automation, this is the method of removing interfering substances from serum .
4. This automated aspect corresponds to sample delivery in the manual method.
5. The normal pH of  blood.
6. The normal bicarbonate and carbonic acid ratio in the body.
7.  The major intracellular cation.
8.  The major extracellular cation.
9.  It is described as the constant concentration at half of the maximum velocity of  enzyme activity.
10. It is the S.I. units of  enzyme activity.
11. Enzyme alcoholic marker.
12. Characterized by elevated bicarbonate, normal PCO2 and abnormal pH.
13. The 3 major cardiac enzymes
14. Same as above
15. Same as above
16. Prostate carcinoma  marker
17. Normal value for AST.
18. Conversion factor for calcium.
19. -20. Formula for Henderson-Hasselbalch equation

 


ANSWERS TO BE POSTED NEXT WEEK.

Read All about FPIA Fluorescence Polarization Immunoassay

• Fluorescein-labeled drug competes with unlabeled drug for antibody

•Sample excited with plane polarized light (490 nm)

•Fluorescein emits plane polarized light (520 nm)

•Small, free drug-fluorescein, rotates faster leading to less emission

•Larger, antibody-drug-fluorescein, rotates slower and emits plane polarized light (520 nm)

Principle: Drug in sample competes for antibody with fluorescein bound drug

•More drug in the sample; less fluorescein labeled drug bound to antibody; lower

emission of plane polarized light.

•Higher drug concentration results in lower light emission values.

FPIA

•Available for a variety of drugs

Advantages

Rapid turnaround times, sensitivity, ease of operation

Disadvantages

Background interference in serum sample (requires blank measurement).

All about polarization