FLUOROMETRY

This method measures the fluorescence of substances in solution and make this as a basis of determining the unknown concentration. This is done by measuring the electromagnetic radiation absorption of excited atoms when they return to a higher energy level than their original levels. The wavelength used will be longer to the excitation wavelength.

Parts of a basic filter fluorometer:

1. Energy Source
2. Primary filter
3. cell or Sample holder
4. Attenuator
5. Secondary filter
6. Detector (Photomultiplier)
7. Readout