ALP catalyzes the hydrolysis of Paranitrophenylphosphate, which is colorless, to paranitrophenol, which is colored yellow, at 405 nanometers.
2. Name differences between ALP and ACP
CATEGORY ALP ACP
pH Basic or alkaline acidic
Best specimen Heparinized plasma (Calbreath) Serum citrated plasma (Calbreath)
Tissue source Same as ACP except for prostate, more on bone
Clin. significance Prostate, platelets, bone, liver, spleen, kidneys, erythrocytes
Diagnostic significance Hepatobiliary and bone disorders Prostatic carcinoma
3. What is the reason for diluting serum if the absorbance is higher than 0.25
For more precise and accurate measurement of the concentration of the unknown.
4. Why do we have to adjust the spectrophotometer to zero when we read unknown solutions?
To read out errors caused by the spectrophotometer and the reagent.
5. What is the best sample for this determination?
Unhemolyzed, clear, non-icteric, non-lipemic serum
